DOI Number :
Hits : 3

Proteins which are Linked with Swiss Webster Mouse Limb Teratogenesis as the Effects of Methoxyacetic Acid (MAA) Treatment

Aceng Ruyani, Sri Sudarwati, Lien A. Sutasurya, Sony H. Sumarsono


Abstract. The analysis of proteins, which are linked with limb teratogenesis as the effects of MAA treated in Swiss Webster mouse has been investigated. A single dose of MAA 10 mmol/kg body weight was given by gavage on gestation day 11, whereas the control group were administered sterilized distilled water. Pregnant mice were sacrificed by cervical dislocation at 4 hours after MAA treatment. The forelimb buds were isolated from both control and treated group embryos and were then homogenized. The crude extracts were then fractionated with ammonium sulfate and each fraction was analyzed by 1-D and 2-D SDS-PAGE techniques respectively. The 1-D and 2-D electrophoregrams revealed that in the treated group of protein fraction 20-40% ammonium sulfate (F-II), a protein of 31.0-36.5 kDa and a protein spot 35.1 kDa, pI 6.2 could be detected, which was not found in the control. In the treated group of protein fraction 40-60% ammonium sulfate (F-III) a protein of 66.3-97.4 kDa and a protein spot 81.7 kDa, pl 7.3 could be detected which was not found in the control, whereas in the control group a protein of 36.5-55.4 kDa, which is a protein spot 41.6 and pI 6.4, was detected but not detected in the treated group. It could be concluded from this experiment hat in the mouse forelimb buds, MAA treatment induce the protein expression of two proteins (35.1 kDa, pI 6.2 and 81.7 kDa, 7.3) and inhibit the expression of one protein (41.6p, pI 6.4).

Keywords: mouse embryo; limb development; methoxyacetic acid; protein profile

Download Article
Bahasa Indonesia | English


Begin on 10 October 2014 this website is no longer activated for article process in Journal of Mathematical and Fundamental Sciences, Journal of Engineering and Technological Sciences, Journal of ICT Research and Applications and Journal of Visual Art and Design. The next process will be proceeded under new website at

For detail information please contact us to:

       ITB Journal Visitor Number #24699472       
       Jl. Tamansari 64, Bandung 40116, Indonesia Visitor IP Address #       
       Tel : +62-22-250 1759 ext. 121 2011 Institut Teknologi Bandung       
       Fax : +62-22-250 4010, +62-22-251 1215 XHTML + CSS + RSS       
       E-mail : or Developed by AVE