DOI Number :
Hits : 25

Effects of Preimplantation Treatments of Rubratoxin B on the Development of Preimplantation Embryos and Fetuses of Swiss Webster Mouse (Mus Musculus)

Ramadhan Sumarmin, Tien W. Surjono & Sri Sudarwati


Abstract. Rubratoxin B is a secondary metabolite of Penicillium rubrum and Penicillium purpurogenum, which moulds are often contaminating cereals, particularly food and feed. Single dosages of rubratoxin B 0.8 and 0.9 mg/kg body weight were administered intraperitoneally to Swiss Webster mice on day 0 or day 2 of gestation (preimplantation stage). Control mice were given propylene glycol only as rubratoxin B solvent. The effects of those treatments on preimplantation embryos were observed on gestation day 3.5, whereas those on fetuses were observed on day 18 of gestation. The results revealed that the development of preimplantation embryos was inhibited, shown by the decreased number of late blastocysts and the presence of earlier developmental stages. In the postimplantation stage these occurred: reduction in the number of implantation sites and live fetuses, increased intrauterine death, and cleft palate in the fetuses. In general, the results of the parameters obtained differed significantly compared to the controls and were dose related. In the treatment on gestation day 0, most of the preimplantation developmental delays occurred at earlier stages, i.e. 1-8 cells stage and uncompacted morulae, whereas in the groups treated on gestation day 2 inhibition shifted to older stages, predominantly at compacted morulae. It is concluded that preimplantation treartments of rubratoxin B inhibit the preimplantation development of the embryos, and consequently decrease the number of implantation sites, as well as the number of live fetuses, and is able to induce fetal malformation.

Keywords: effects of rubratoxin B; embryos; fetuses; malformation; mouse; preimplantation treatment

Download Article
Bahasa Indonesia | English


Begin on 10 October 2014 this website is no longer activated for article process in Journal of Mathematical and Fundamental Sciences, Journal of Engineering and Technological Sciences, Journal of ICT Research and Applications and Journal of Visual Art and Design. The next process will be proceeded under new website at

For detail information please contact us to:

       ITB Journal Visitor Number #24508914       
       Jl. Tamansari 64, Bandung 40116, Indonesia Visitor IP Address #       
       Tel : +62-22-250 1759 ext. 121 2011 Institut Teknologi Bandung       
       Fax : +62-22-250 4010, +62-22-251 1215 XHTML + CSS + RSS       
       E-mail : or Developed by AVE