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The Uptake of 32P by Brown Planthoppers (Nilaparvata lugens Stal) Gonads

S. Sastrodihardjo & R.A. Wahid


Abstract. The radioisotop 32P has been used quite extensively in the field of insect ecology and insect physiology. It has been widely known that 32P is one of the several isotopes applied to tag the insects for migration studies, to estimate the sap uptake or the excretion of sucking insects as well as for the study of sperms transfer in sterilization study. Within the scope of Sterile Insects Techniques, the study on the uptake of 32P by the gonads of brown planthoppers (Nilaparvata lugens Stal) could be meaningful. The 32P radioisotopes used had been one of most available products of the Centre. The radioisotope provided also a possible source for sterilization due to its accumulative effect. Results of the investigation on the 32P uptake by the whole body and gonads after feeding on radioactive rice seedlings will be reported.
a. Absorption of 32P by rice seedling ( Pelita I/1 )
The specific activities of 32P (Carrier free) applied to the two week old seedlings were 1, 2, 4, 6, 8, 10, 15, and 20 uci/cc. The radioactivity count of hoppers and leaf tips were made 48 hrs. after submersion of the seedlings in the radioactive solution.
b. Absorption of 32P by BPH
The radioactive seedlings were offered to BPH after being washed thoroughly with water. A single BPH is fed on a single rice seedlings kept in a glass tube. Count of the whole body and dissected gonads were made 24, 48, 72, and 96 hours after feeding. Sterility effects were observed from the mating of 32P treated hoppers.


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