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Pembuatan Sediaan 99mtc-Sel Darah Merah sebagai Penyidik Limpa

Dr. A. Hanafiah Ws



Labeling of red blood cells with 99mTc radionuclide was carried out using SnCl2 solution as a reductor. The optimum required condition for rat blood was obtained in a solution containing 1 μg SnCl2.2H2O which was heated for 15 minutes at 49-500C. Labeling efficiency and radiochemical purity of radiolabeled compound was determined by ascending paper chromatography using Whatman No. I paper as stationary phase and methanol 85% as mobile phase. The result of biodistribution and imaging in the Wistar white rat revealed that the preparation was accumulated in the spleen.


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