DOI Number : 10.5614/
Hits : 15

Optimization of Fermented Tofu with High Isoflavone Content through Variation of Inoculum Percentages and Ratios of Lactobacillus plantarum, Lactobacillus acidophilus, and Leuconostoc mesenteroides

Dea Indriani Astuti & Zahra Noviana

Microbial Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia

Abstract. Tofu is a traditional food that can be found in several countries, such as Indonesia and Japan. Tofu is often made using the backslopping method, which brings the risk of pathogenic microorganism contamination and can lead to failure in production. Standardization of the production process is necessary in order to yield highly nutritious tofu that is safe to be consumed. In this research, standardization was investigated by variation of the inoculum percentages and ratios of Lactobacillus plantarum (a), Lactobacillus acidophilus (b), and Leuconostoc mesenteroides (c). The inoculum percentages used in this research were 7.5%, 10%, and 12.5%, while the inoculum ratios between a, b, and c were (a:b:c) 2:1:1; 1:2:1; 1:1:2; and 1:1:1. The optimum inoculum ages for a, b, and c were 8, 6, and 2 hours respectively. The highest growth rates of a, b, and c were 0.060h-1, 0.054h-1, and 0.092h-1 respectively. The highest lactic acid production rates of a, b, and c were 0.072%h-1, 0.063%h-1, and 0.126%h-1 respectively. The inoculum percentage and ratio that produced the highest isoflavones content was 12.5%(2:1:1), with the highest bacterial population growth rate being 0.019h-1, the highest formation rate of lactic acid being 0.045%h-1, and the isoflavone aglycones level being 0.445 mg/mL.

Keywords: fermentation; isoflavone; lactic acid; lactic acid bacteria; tofu.

Download Article
Bahasa Indonesia | English


Begin on 10 October 2014 this website is no longer activated for article process in Journal of Mathematical and Fundamental Sciences, Journal of Engineering and Technological Sciences, Journal of ICT Research and Applications and Journal of Visual Art and Design. The next process will be proceeded under new website at

For detail information please contact us to:

       ITB Journal Visitor Number #9626092       
       Jl. Tamansari 64, Bandung 40116, Indonesia Visitor IP Address #       
       Tel : +62-22-250 1759 ext. 121 2011 Institut Teknologi Bandung       
       Fax : +62-22-250 4010, +62-22-251 1215 XHTML + CSS + RSS       
       E-mail : or Developed by AVE