DOI Number : 10.5614/itbj.eng.sci.2006.38.1.1
Hits : 9

Ribotyping Identification of Thermophilic Bacterium from Papandayan Crater

Akhmaloka, A. Suharto, S. Nurbaiti, I N. Tika & F. M. Warganegara

Biochemistry Research Division, Faculty of Mathematics and Natural Sciences,

Institut Teknologi Bandung,

Jl Ganesha 10 Bandung, Indonesia, email: loka@chem.itb.ac.id


Abstract.

A few thermophilic bacteria were isolated from a hot spring located in Papandayan Crater, Garut. One of the organisms showed a well growth at temperature of up to 80oC. Chromosomal DNA from the organism was isolated and used to amplify 16S rRNA gene fragment. The gene was amplified by a set of universal primers (27F and 1492R) resulting in a 1.5 kb DNA fragment. The gene was cloned and sequenced. The phylogenetic tree, homological analysis, and detailed comparison of the sequences showed that 16S rRNA gene sequence of the Papandayan isolate is unique compared to other known strains, however the sequence had closest similarities with Bacillus caldolyticus and Bacillus caldotenax.



Keywords: Bacillus caldolyticus; Bacillus caldotenax; Papandayan crater; thermophilic microorganism; 16S rRNA gene.

Download Article
 
Bahasa Indonesia | English
 
 
 

Notification:

Begin on 10 October 2014 this website is no longer activated for article process in Journal of Mathematical and Fundamental Sciences, Journal of Engineering and Technological Sciences, Journal of ICT Research and Applications and Journal of Visual Art and Design. The next process will be proceeded under new website at http://journals.itb.ac.id.

For detail information please contact us to: journal@lppm.itb.ac.id.

 
       
       
       ITB Journal Visitor Number #14981564       
       Jl. Tamansari 64, Bandung 40116, Indonesia Visitor IP Address #       
       Tel : +62-22-250 1759 ext. 121 2011 Institut Teknologi Bandung       
       Fax : +62-22-250 4010, +62-22-251 1215 XHTML + CSS + RSS       
       E-mail : journal@lppm.itb.ac.id or proceedings@lppm.itb.ac.id Developed by AVE