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Mechanism of Action of Coumarin against Candida albicans by SEM/TEM Analysis
Gunawan Pamudji Widodo1, Elin Yulinah Sukandar2, I Ketut Adnyana2 & Sukrasno3
1Faculty of Pharmacy, Universitas Setia Budi, Surakarta 57127, Indonesia
2Pharmacology-Clinical Pharmacy, School of Pharmacy, Institut Teknologi Bandung, Bandung 40132, Indonesia
3Biological Chemistry, School of Pharmacy, Institut Teknologi Bandung, Bandung 40132, Indonesia
Email: gunawanpamudji@yahoo.com
Abstract. The aim of this study was to identify the antifungal activity of coumarin isolated from Ageratum conyzoides L. leaves and to observe its influence on Candida albicans cells by scanning electron microscope (SEM) and transmission electron microscope (TEM). Antifungal activity testing by disk diffusion method showed coumarin was active toward pathogenic fungus, Candida albicans with the MIC value of coumarin of 125 g mL-1. The influence of this substance on C. albicans cells was observed by scanning and transmission electron microscopies. The result showed that this compound damaged the cell by pores formation on the cell wall. The death of cells occurred due to leakage and necrotic of cytoplasmic content.
Keywords: Ageratum leaves; antifungal activity; Candida albicans; coumarin; SEM; TEM.
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