DOI Number : 10.5614/itbj.eng.sci.2012.44.2.1
Hits : 21

Optimization of Laccase Production using White Rot Fungi and Agriculture Wastes in Solid State Fermentation

Hendro Risdianto1, Elis Sofianti2, Sri Harjati Suhardi3, Tjandra Setiadi2

1Center for Pulp and Paper, Ministry of Industry
Jl. Raya Dayeuhkolot 132, Bandung, Jawa Barat 40258, Indonesia
2Dept. of Chemical Engineering, Institut Teknologi Bandung, Labtek X, Jl. Ganesa 10, Bandung, Jawa Barat 40132, Indonesia
 3School of Life Sciences and Technology, Institut Teknologi Bandung, Labtek XI, Jl. Ganesha 10, Bandung, Jawa Barat 40132, Indonesia
Email : hendrorisdianto@yahoo.com


Abstract. Laccase has been produced in a solid state fermentation (SSF) using white rot fungi and various lignocellulosic based substrates. White rot fungi used were Marasmius sp, Trametes hirsuta, Trametes versicolor and Phanerochaete crysosporium. The solid substrates employed in this research were collected from agriculture waste which were empty fruit bunches (EFB), rice straw, corn cob, and rice husk. The objective of this research was to determine the most promising fungus, the best solid substrate and the optimal conditions for the production of laccase. The results showed that Marasmius sp. on all solid substrates displayed higher laccase activity than that of any other strain of white rot fungi. Marasmius sp. and solid substrate of rice straw demonstrated the highest laccase activity of 1116.11 U/L on day 10. Three significant factors, i.e. pH, temperature and yeast extract concentration were studied by response surface method on laccase production using Marasmius sp and rice straw. The optimized conditions were pH, temperature and yeast extract concentration of 4.9, 31șC and 0.36 g/L respectively. The fermentation of Marasmius sp. in SSF on agricultural waste shows a great potential for the production of laccase.

Keywords: agriculture waste; laccase; Marasmius sp.; solid state fermentation; optimisation.

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