Induction of Somatic Embryos in Cultured Leaf Explants of Coffea Arabica

E. Noerhadi, T. Yasuda, Arbayah Siregar & Risco G. Budji

Somatic embryo induction and subsequent plantlets development in culture of Coffea arabica leaf tissue explants was described. For callus formation leaf segments were grown on medium Linsmaier and Skoog with 3% sucrose and varying concentrations of 2,4-Dichlorophenoxyaceticacid or Naphthalene Acetic Acid and Kinetin. Eighteen weeks after inoculation, callus will be formed on Linsmaier and Skoog's medium with 2 μM 2,4-Dichlorophenoxyacetic acid and 5-7 μM Kinetin and also on medium with 0.05 μM Naphtalene Acetic Acid and 6-8 μM Kinetin. Somatic embryos are then formed by inoculation of calli segments on medium Linsmaier & Skoog with 3 μM to 35 μM Kinetin and 0.05 μM Indole Butyric Acid. Somatic embryos are also formed in liquid medium of Gamborg, Miller and Ojima supplemented with 0.05 μM to 2.5 μM 2,4-Dichlorophenoxyacetic acid.

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