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Increase of Oxidative Stress and Accumulation of α-Synuclein in Wistar Rat’s Midbrain Treated with Rotenone
Arief Budi Yulianti1, Sony Heru Sumarsono2, Ahmad Ridwan2 & Ayda T. Yusuf2
1Medical Biology, Faculty of Medicine, Bandung Islamic University,
Jalan Hariangbanga No. 2, Bandung 40116, Indonesia
2Research Group of Physiology, Developmental Biology and Biomedical Science, School of Life Science and Technology, Bandung Institute of Technology
Jalan Ganesha No. 10, Bandung 40132, Indonesia
Email: iyoelfk@yahoo.ca; budi.yulifk@gmail.com
Abstract. A neurodegenerative disorder caused by oxidative stress leads to an accumulation of α-synuclein on neurons. Here, we report our findings on the effect of rotenone on the increase of oxidative stress and accumulation of α-synuclein in the midbrain of Wistar rats. Thirty six male rats (8–9 weeks, 200-250 g) were divided into three groups: the Blank group, the Solvent-rotenone group (intraperitoneally injected with 1 ml/kg body weight of sunflower seed oil), and the Rotenone group (intraperitoneally injected with 2.5 mg/kg body weight of rotenone) for 9, 19, and 28 days. The rats were decapitated on day 10 for the 9-day treatment, day 20 for the 19-day treatment, day 30, 40, 50 and 60 for the 28-day treatment (2 rats/group or 6 rats/day of observation time). The midbrains were isolated and extracted. Glutathione assay and α-synuclein ELISA tests were performed. The results showed that the average oxidative stress index was highest in the Blank group (0.95 ± 0.24, 0.63 ± 0.23, 0.81 ± 0.27, respectively). Meanwhile, the concentration of α-Synuclein had decreased in all groups (0.09 ± 0.03, 0.15 ± 0.03, and 0.13 ± 0.02 ng/mg tissue, respectively). Correlation analysis showed that the oxidative stress index was inversely proportional to the concentration of α-synuclein. Our conclusion is that the midbrain of Wistar rats treated with rotenone indicated oxidative stress and led to an accumulation of α-synuclein protein.
Keywords: α-synuclein; antioxidant; neurodegeneration; oxidative stress; rotenone.
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