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Differential Expression of Vimentin and GFAP Protein during Brain Development of Mouse Fetuses after Treated with 2-Methoxyethanol
Yulia Irnidayanti1, Win Darmanto2, Agus Abadi3, Yukio Hattori4 & Yasuhiro Yamashiro4
1Biology Department, UNJ, Faculty of Mathematics and Science,
Jalan Pemuda No. 10, Jakarta 13150, Indonesia
2Biology Departement Faculty of Mathematics and Science, Airlangga University,
Mulyorejo Kampus C Surabaya 60115, Indonesia
3Faculty of Medicine, Airlangga University,
Jalan Mayjen Prof. Dr. Moestopo 47, Surabaya, Indonesia 60131
4Division of Clinical Laboratory Science, Faculty of Health Sciences, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube,
Yamaguchi 755-8505, Japan
Email: irnidayanti@unj.ac.id
Abstract. This study aims to determine the differences on protein expression of vimentin and GFAP in the brains of fetal mice Mus musculus (Swiss Webster) during the period of brain development ( at the GD-11 to the GD-18), caused by 2-methoxyethanol (2ME) that injected intraperitoneally with a dose of 7,5 mmol/kg body weight at gestation days (GD) 10. In the last decade, it has been widely reported the negative effects of chemicals polluting the environment. 2-methoxyethanol is one of the chemical pollutants, which is teratogenic, embryotoxic or teratogenic in both animals and humans. 2-ME and its metabolites in the body can damage cells and tissues. The body can be exposed by 2-ME through the air, water and soil. Fetal brains were isolated and collected, into the tube containing a solution of Cell Lytic M reagent. Protein concentration was measured by BCA nanodrops 2000 and the Smart Kit Cat No. 21071 Japan. To determine the protein expression immunodot blot was performed by using primary antibodies vimentin and GFAP. The amount of protein expressed was measured by immnodotblot and densitometry techniques.. The results showed that the expression of vimentin was always higher than GFAP in the brains of fetal at GD-11 until GD-18. The highest expression of vimentin was highest in fetal brain at GD-17 and18 days. The highest expression of GFAP present in fetal brain GD-12. Generally, it could be concluded that the protein Vimentin is very necessary during the period of fetal brain development, especially for healing wounds caused by 2-Methoxyethanol. Vimentin, GFAP, Malformation of brain, 2-Methoxyethanol.
Keywords: brain; cell repair; disruption; GFAP; proliferation; protein translation; cell response; 2-Methoxyethanol; Vimentin.
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